6QQA
Room temperature structure of the fluorescent protein Cerulean recorded after an accumulated dose of 21 kGy
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 293 |
| Detector technology | PIXEL |
| Collection date | 2014-12-01 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.976 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.930, 63.040, 71.320 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.230 - 1.660 |
| R-factor | 0.13709 |
| Rwork | 0.135 |
| R-free | 0.16713 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.537 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0218) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.240 | 1.700 |
| High resolution limit [Å] | 1.660 | 1.660 |
| Number of reflections | 28306 | 2062 |
| <I/σ(I)> | 15.43 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.3 | 7.4 |
| CC(1/2) | 0.998 | 0.753 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 15% PEG 8000, 0.1M MGCL2, 0.1 M HEPES PH 7.0 |
