6QQA
Room temperature structure of the fluorescent protein Cerulean recorded after an accumulated dose of 21 kGy
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 293 |
| Detector technology | PIXEL |
| Collection date | 2014-12-01 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.976 |
| Spacegroup name | P 21 21 21 |
| 格子定数 [Å] | 51.930, 63.040, 71.320 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 47.230 - 1.660 |
| R因子 | 0.13709 |
| Rwork | 0.135 |
| R-free | 0.16713 |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.011 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.537 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0218) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 47.240 | 1.700 |
| 分解能 [Å] (高) | 1.660 | 1.660 |
| 独立反射数 | 28306 | 2062 |
| <I/σ(I)> | 15.43 | |
| 完全性 [%] | 100.0 | 100 |
| 冗長性 | 7.3 | 7.4 |
| CC(1/2) | 0.998 | 0.753 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 15% PEG 8000, 0.1M MGCL2, 0.1 M HEPES PH 7.0 |
