6QM2
NlaIV restriction endonuclease
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
Synchrotron site | MPG/DESY, HAMBURG |
Beamline | BW6 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-12-17 |
Detector | MARRESEARCH |
Wavelength(s) | 1.05 |
Spacegroup name | P 62 2 2 |
Unit cell lengths | 110.380, 110.380, 209.006 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.720 - 2.800 |
R-factor | 0.19245 |
Rwork | 0.191 |
R-free | 0.22103 |
Structure solution method | MAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.083 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | autoSHARP |
Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.970 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.043 | 1.052 |
Number of reflections | 18997 | |
<I/σ(I)> | 28.92 | 1.72 |
Completeness [%] | 98.5 | 98.6 |
Redundancy | 7.5 | 7.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5 | 294 | 10 mg/ml of the enzyme in 15 % glycerol, 50 mM NaOH/HEPES pH 7,5, 50 mM KCl, 10 mM DTT and 5 mM CaCl2 was mixed in 1:1 ratio with double stranded DNA composed of the 5'-ATGGTACCTGC-3' and 5'-CAGGTACCATG-3' strands. The protein-DNA solutions were in turn mixed in 1:1 ratio with the precipitant solution containing 2 M NaCl and 100 mM citric acid, pH 5. |