6Q5Q

Crystal structure of a CC-Hex mutant that forms an antiparallel four-helix coiled coil CC-Hex-KgEb

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	80
Detector technology	CCD
Collection date	2010-05-17
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9795
Spacegroup name	I 2 2 2
格子定数 [Å]	28.570, 29.350, 55.722
格子定数 [度]	90.00, 90.00, 90.00

精密化法

残基	16.500 - 1.080
R因子	0.13531
Rwork	0.135
R-free	0.14659
Structure solution method	AB INITIO PHASING
結合長の平均二乗偏差(RMSD) [Å]	0.021
結合角の平均二乗偏差(RMSD) [度]	2.222
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	ACORN
Refinement software	REFMAC (5.8.0238)

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	20.470	1.140
分解能 [Å] (高)	1.080	1.080
Rmerge_l_obs	0.074	0.706
Rmeas	0.077	0.737
Rpim	0.021
独立反射数	10348	1461
<I/σ(I)>	17.5	56.2
完全性 [%]	99.7	94.7
冗長性	12.8	12.2
CC(1/2)	0.999	0.943

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, SITTING DROP		298	0.2M potassium/sodium tartrate tetrahydrate 20% w/v PEG 3350.