6Q5O
Crystal structure of a CC-Hex mutant that forms an antiparallel four-helix coiled coil CC-Hex*-LL
Experimental procedure
実験手法 | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2018-10-22 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.77999 |
Spacegroup name | I 2 2 2 |
格子定数 [Å] | 28.868, 29.378, 55.119 |
格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
残基 | 27.560 - 1.000 |
R因子 | 0.17017 |
Rwork | 0.168 |
R-free | 0.21193 |
Structure solution method | MOLECULAR REPLACEMENT |
結合長の平均二乗偏差(RMSD) [Å] | 0.012 |
結合角の平均二乗偏差(RMSD) [度] | 1.816 |
Data reduction software | XDS (BUILT20180808) |
Data scaling software | Aimless (0.7.2) |
Phasing software | PHASER (2.8.2) |
Refinement software | REFMAC (5.8.0238) |
Quality characteristics
Overall | Inner shell | Outer shell | |
分解能 [Å] (低) | 27.560 | 27.560 | 1.017 |
分解能 [Å] (高) | 1.000 | 2.714 | 1.000 |
Rmerge_l_obs | 0.050 | 0.034 | 4.401 |
Rmeas | 0.054 | 0.037 | 4.769 |
Rpim | 0.021 | 0.014 | 1.826 |
独立反射数 | 13041 | 721 | 643 |
<I/σ(I)> | 15.5 | 61.8 | 0.4 |
完全性 [%] | 99.9 | 99.6 | 100 |
冗長性 | 12.5 | 11.4 | 12.8 |
CC(1/2) | 0.999 | 0.999 | 0.450 |
結晶化条件
結晶ID | 方法 | pH | 温度 | 溶液条件 |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 0.6 M potassium sodium tartrate tetrahydrate, 50 mM Tris |