6Q5K
Crystal structure of a CC-Hex mutant that forms an antiparallel four-helix coiled coil CC-Hex*-L24K
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2013-04-28 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 0.92 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 69.280, 69.280, 35.441 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 24.780 - 1.650 |
R-factor | 0.17395 |
Rwork | 0.172 |
R-free | 0.21834 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.013 |
RMSD bond angle | 1.856 |
Data reduction software | MOSFLM |
Data scaling software | Aimless (0.7.1) |
Phasing software | PHASER (7.0.06) |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 24.780 | 24.780 | 1.680 |
High resolution limit [Å] | 1.650 | 9.040 | 1.650 |
Rmerge | 0.071 | 0.029 | 1.356 |
Rmeas | 0.073 | 0.030 | 1.393 |
Rpim | 0.018 | 0.008 | 0.316 |
Number of reflections | 6134 | 53 | 269 |
<I/σ(I)> | 17.6 | 50.4 | 2.7 |
Completeness [%] | 96.5 | 95.4 | 100 |
Redundancy | 20.4 | 14.5 | 18.8 |
CC(1/2) | 1.000 | 0.682 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 297 | 0.05 M Tris, 0.75 M ammonium sulfate |