6Q5J
Crystal structure of a CC-Hex mutant that forms a parallel six-helix coiled coil CC-Hex*-L24E
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 77 |
| Detector technology | PIXEL |
| Collection date | 2018-03-04 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97628 |
| Spacegroup name | P 42 21 2 |
| 格子定数 [Å] | 55.073, 55.073, 138.940 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 43.190 - 1.690 |
| R因子 | 0.21813 |
| Rwork | 0.216 |
| R-free | 0.25792 |
| Structure solution method | MOLECULAR REPLACEMENT |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.013 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.618 |
| Data reduction software | DIALS (1.8.5-g9e40d7de) |
| Data scaling software | Aimless (0.5.32) |
| Phasing software | PHASER (2.8.2) |
| Refinement software | REFMAC (5.8.0230) |
Quality characteristics
| Overall | Inner shell | Outer shell | |
| 分解能 [Å] (低) | 46.320 | 46.320 | 1.730 |
| 分解能 [Å] (高) | 1.690 | 7.560 | 1.690 |
| Rmerge_l_obs | 0.193 | 0.041 | 1.154 |
| Rmeas | 0.197 | 0.042 | 1.178 |
| Rpim | 0.011 | 0.235 | |
| 独立反射数 | 24910 | 7200 | 1809 |
| <I/σ(I)> | 10.6 | ||
| 完全性 [%] | 100.0 | 99.7 | 100 |
| 冗長性 | 24.5 | 19.7 | 24.6 |
| CC(1/2) | 1.000 | 1.000 | 0.505 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 100 mM NaCl, 50 mM MES buffer and 10% w/v PEG 2000 MME at pH 6.0 |
