6Q5I
Crystal structure of a CC-Hex mutant that forms an antiparallel four-helix coiled coil CC-Hex*-L24E
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2018-03-04 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97628 |
| Spacegroup name | P 61 2 2 |
| 格子定数 [Å] | 69.070, 69.070, 35.490 |
| 格子定数 [度] | 90.00, 90.00, 120.00 |
精密化法
| 残基 | 17.380 - 1.760 |
| R因子 | 0.1843 |
| Rwork | 0.183 |
| R-free | 0.20731 |
| Structure solution method | MOLECULAR REPLACEMENT |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.014 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.781 |
| Data reduction software | DIALS (1.8.5-g9e40d7de-release) |
| Data scaling software | Aimless (0.5.32) |
| Phasing software | PHASER (2.8.2) |
| Refinement software | REFMAC (5.8.0238) |
Quality characteristics
| Overall | Inner shell | Outer shell | |
| 分解能 [Å] (低) | 17.380 | 17.380 | 1.810 |
| 分解能 [Å] (高) | 1.760 | 7.870 | 1.760 |
| Rmerge_l_obs | 0.205 | 0.051 | 4.738 |
| Rmeas | 0.208 | 0.052 | 4.800 |
| Rpim | 0.034 | 0.010 | 0.763 |
| 独立反射数 | 5281 | 75 | 382 |
| <I/σ(I)> | 12.9 | 52.4 | 1 |
| 完全性 [%] | 100.0 | 91.2 | 100 |
| 冗長性 | 36.5 | 27.3 | 39.2 |
| CC(1/2) | 0.999 | 0.999 | 0.661 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 1.0 M Ammonium Sulphate and 50 mM Tris |
