Loading
PDBj
MenuPDBj@FacebookPDBj@TwitterPDBj@YouTubewwPDB FoundationwwPDB
RCSB PDBPDBeBMRBAdv. SearchSearch help

6OJS

Crystal structure of TS-DHFR from Cryptosporidium hominis in complex with NADPH, FdUMP, MTX and 2-amino-4-oxo-4,7-dihydro-pyrrolo[2,3-d]pyrimidine-methyl-phenyl-D-glutamic acid

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsAPS BEAMLINE 24-ID-E
Synchrotron siteAPS
Beamline24-ID-E
Temperature [K]100
Detector technologyPIXEL
Collection date2018-07-06
DetectorDECTRIS EIGER X 16M
Wavelength(s)0.97915
Spacegroup nameC 1 2 1
Unit cell lengths212.622, 116.188, 220.884
Unit cell angles90.00, 95.48, 90.00
Refinement procedure
Resolution50.121 - 3.214
R-factor0.2186
Rwork0.218
R-free0.23170
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)4q0d
RMSD bond length0.003
RMSD bond angle0.502
Data reduction softwareXDS
Data scaling softwareXDS
Phasing softwarePHASER
Refinement softwarePHENIX ((1.10.1_2155))
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]51.0003.410
High resolution limit [Å]3.2103.210
Number of reflections8585112774
<I/σ(I)>4.981.06
Completeness [%]98.291.1
Redundancy6.96.7
CC(1/2)0.9830.616
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP8295.15Well solution 22% PEG 6000, 0.2 M ammonium sulfate, 0.06 M lithium sulfate, 0.1 M Tris Drop ratio 2:1 Enzyme mix/well solution

225158

數據於2024-09-18公開中

PDB statisticsPDBj update infoContact PDBjnumon