6NIB
Crystal Structure of Medicago truncatula Agmatine Iminohydrolase (Deiminase)
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-08-04 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1.00 |
| Spacegroup name | C 1 2 1 |
| 格子定数 [Å] | 146.996, 75.504, 47.103 |
| 格子定数 [度] | 90.00, 108.56, 90.00 |
精密化法
| 残基 | 34.695 - 1.200 |
| R因子 | 0.112 |
| Rwork | 0.112 |
| R-free | 0.13670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1vkp |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.014 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.397 |
| Data reduction software | XDS (VERSION Jan 26, 2018) |
| Data scaling software | STARANISO |
| Phasing software | PHASER (2.8.2) |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 39.960 | 1.280 |
| 分解能 [Å] (高) | 1.200 | 1.200 |
| Rmerge_l_obs | 0.031 | 0.463 |
| Rmeas | 0.036 | 0.560 |
| Rpim | 0.019 | 0.309 |
| 独立反射数 | 121248 | 5584 |
| <I/σ(I)> | 19.1 | 2.5 |
| 完全性 [%] | 93.8 | 58.5 |
| 冗長性 | 3.63 | 3.12 |
| CC(1/2) | 1.000 | 0.758 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 292 | 0.2 M Sodium Acetate, 20% PEG 3350, cryoprotected with 25% Glycerol |
