6NC7
Lipid II flippase MurJ, inward open conformation
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-06-29 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.98 |
| Spacegroup name | C 1 2 1 |
| 格子定数 [Å] | 129.692, 105.514, 111.479 |
| 格子定数 [度] | 90.00, 125.29, 90.00 |
精密化法
| 残基 | 90.992 - 3.000 |
| R因子 | 0.2499 |
| Rwork | 0.249 |
| R-free | 0.27110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5t77 |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.005 |
| 結合角の平均二乗偏差(RMSD) [度] | 0.679 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 100.000 | 3.100 |
| 分解能 [Å] (高) | 3.000 | 3.000 |
| 独立反射数 | 20396 | |
| <I/σ(I)> | 11.41 | |
| 完全性 [%] | 99.0 | |
| 冗長性 | 5.5 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | LIPIDIC CUBIC PHASE | 295 | PEG400, ammonium sulfate |
