6K18

Crystal structure of EXD2 exonuclease domain soaked in Mn

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-10-31
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97950
Spacegroup name	P 21 21 21
格子定数 [Å]	46.217, 73.866, 131.683
格子定数 [度]	90.00, 90.00, 90.00

精密化法

残基	43.609 - 2.303
R因子	0.2135
Rwork	0.210
R-free	0.27130
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6k17
結合長の平均二乗偏差(RMSD) [Å]	0.003
結合角の平均二乗偏差(RMSD) [度]	0.645
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	50.000	2.340
分解能 [Å] (高)	2.300	2.300
Rmerge_l_obs	0.103	0.348
独立反射数	20317	939
<I/σ(I)>	12.35
完全性 [%]	98.3
冗長性	5.2

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, HANGING DROP		277	PEG 8000, 2-(N-morpholino)ethanesulfonic acid, Calcium acetate, Dithiothreitol, Manganese chloride