6JJ1
Crystal structure of peptidyl-tRNA hydrolase from Acinetobacter baumannii at 0.97 A resolution with disordered five N-terminal residues
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-12-01 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97199 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 33.986, 66.131, 76.103 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.080 - 0.970 |
R-factor | 0.13418 |
Rwork | 0.134 |
R-free | 0.16705 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5y9a |
RMSD bond length | 0.020 |
RMSD bond angle | 2.006 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.080 | 1.000 |
High resolution limit [Å] | 0.970 | 0.970 |
Rpim | 0.028 | 0.629 |
Number of reflections | 94081 | 3426 |
<I/σ(I)> | 14.9 | |
Completeness [%] | 92.0 | |
Redundancy | 8.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 12% PEG 1500, 0.1M HEPES, pH 7.5, 20% Glycerol |