6JGJ
Crystal structure of the F99S/M153T/V163A/E222Q variant of GFP at 0.78 A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 56 |
Detector technology | PIXEL |
Collection date | 2016-06-16 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.35 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 50.870, 62.340, 68.850 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 31.140 - 0.780 |
Rwork | 0.108 |
R-free | 0.12500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2wur |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | SHELXL |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 31.140 | 31.140 | 0.800 |
High resolution limit [Å] | 0.770 | 3.440 | 0.780 |
Rmerge | 0.073 | 0.039 | 2.098 |
Rmeas | 0.076 | 0.040 | 2.229 |
Number of reflections | 254665 | 3127 | 22721 |
<I/σ(I)> | 15.68 | 71.78 | 1.12 |
Completeness [%] | 98.9 | 99.7 | 98 |
Redundancy | 11.873 | 13.071 | 8.63 |
CC(1/2) | 0.999 | 0.998 | 0.507 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8.5 | 308 | PEG 4000, MgCl2, Tris-HCl buffer |