6INT
xylose isomerase from Paenibacillus sp. R4
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 5C (4A) |
| Synchrotron site | PAL/PLS |
| Beamline | 5C (4A) |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2018-07-28 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9796 |
| Spacegroup name | P 21 21 21 |
| 格子定数 [Å] | 89.282, 124.039, 315.001 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 23.834 - 1.942 |
| R因子 | 0.2068 |
| Rwork | 0.204 |
| R-free | 0.24920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1a0c |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.007 |
| 結合角の平均二乗偏差(RMSD) [度] | 0.812 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.14_3260: ???)) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 50.000 | 1.980 |
| 分解能 [Å] (高) | 1.940 | 1.940 |
| 独立反射数 | 256014 | |
| <I/σ(I)> | 42.9 | |
| 完全性 [%] | 99.8 | |
| 冗長性 | 13.7 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2M calcium chloride, 0.1M HEPES: NaOH, pH 7.5, 27%(v/v) PPG P400 |
