6HIU
Cytochrome P460 from Methylococcus capsulatus (Bath)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-03-12 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.928 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 46.623, 81.647, 85.264 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.670 - 1.360 |
R-factor | 0.1969 |
Rwork | 0.197 |
R-free | 0.22370 |
Structure solution method | SAD |
RMSD bond length | 0.021 |
RMSD bond angle | 1.840 |
Data reduction software | xia2 |
Data scaling software | Aimless |
Phasing software | Auto-Rickshaw |
Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.620 | 1.380 |
High resolution limit [Å] | 1.360 | 1.360 |
Rmerge | 0.043 | 1.048 |
Rmeas | 0.048 | 1.195 |
Rpim | 0.020 | 0.562 |
Number of reflections | 70585 | 3431 |
<I/σ(I)> | 13.7 | 1.2 |
Completeness [%] | 99.9 | 98.5 |
Redundancy | 5.4 | 4.4 |
CC(1/2) | 1.000 | 0.486 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8 | 291 | 2microlitres of 15mg/ml protein in 20mM Tris buffer, pH8, with an equivalent volume of reservoir solution containing 0.1M Tris, 2.4M Ammonium sulfate pH8 |