6HIH
Cytochrome c prime beta from Methylococcus capsulatus (Bath)
Experimental procedure
実験手法 | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-12-11 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97 |
Spacegroup name | P 21 3 |
格子定数 [Å] | 105.960, 105.960, 105.960 |
格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
残基 | 61.200 - 1.600 |
R因子 | 0.17798 |
Rwork | 0.177 |
R-free | 0.20233 |
Structure solution method | SAD |
結合長の平均二乗偏差(RMSD) [Å] | 0.015 |
結合角の平均二乗偏差(RMSD) [度] | 2.154 |
Data reduction software | xia2 |
Data scaling software | Aimless (0.5.17) |
Phasing software | CRANK2 |
Refinement software | REFMAC (5.8.0230) |
Quality characteristics
Overall | Outer shell | |
分解能 [Å] (低) | 61.200 | 1.640 |
分解能 [Å] (高) | 1.600 | 1.600 |
Rmerge_l_obs | 0.047 | 0.930 |
Rmeas | 0.051 | 1.070 |
Rpim | 0.018 | 0.510 |
独立反射数 | 52377 | 3805 |
<I/σ(I)> | 20.9 | 1.4 |
完全性 [%] | 99.0 | 99.4 |
冗長性 | 7.6 | 4 |
CC(1/2) | 0.999 | 0.540 |
結晶化条件
結晶ID | 方法 | pH | 温度 | 溶液条件 |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 15 mg/ml protein mixed with 10 mM ZnSO4, 35% PEG 550, 100 mM MES pH 6.5 |