6H24
X-Ray Crystal Structure of the MSBI1.176 WH1 Domain, a Replication Protein Isolated from a Multiple Sclerosis Patient
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-12-04 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.85001 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 32.380, 77.770, 47.680 |
| Unit cell angles | 90.00, 90.66, 90.00 |
Refinement procedure
| Resolution | 40.650 - 1.530 |
| R-factor | 0.1868 |
| Rwork | 0.185 |
| R-free | 0.21160 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.737 |
| Data reduction software | XDS (VERSION Jan 26, 2018) |
| Data scaling software | XSCALE (VERSION Jan 26, 2018) |
| Phasing software | HKL2Map |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.650 | 1.580 |
| High resolution limit [Å] | 1.530 | 1.530 |
| Rmerge | 0.041 | 0.685 |
| Rmeas | 0.044 | 0.749 |
| Rpim | 0.017 | 0.297 |
| Number of reflections | 35709 | 3473 |
| <I/σ(I)> | 20.91 | 1.98 |
| Completeness [%] | 99.4 | 96.93 |
| Redundancy | 6.4 | |
| CC(1/2) | 1.000 | 0.850 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.2 M magnesium acetate, 20% PEG3350 | |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.2 M magnesium acetate, 20% PEG3350 | |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.2 M magnesium acetate, 20% PEG3350 |
