6H0K
Hen egg-white lysozyme structure determined with data from the EuXFEL, the first MHz free electron laser, 7.47 keV photon energy
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | FREE ELECTRON LASER |
| Source details | EUROPEAN XFEL BEAMLINE SPB/SFX |
| Synchrotron site | European XFEL |
| Beamline | SPB/SFX |
| Temperature [K] | 293 |
| Detector technology | PIXEL |
| Collection date | 2018-06-14 |
| Detector | AGIPD |
| Wavelength(s) | 1.66 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 79.850, 79.850, 38.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.710 - 2.200 |
| R-factor | 0.2005 |
| Rwork | 0.196 |
| R-free | 0.23960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6gf0 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.619 |
| Data reduction software | CrystFEL |
| Data scaling software | CrystFEL |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.800 | 2.300 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 6717 | |
| <I/σ(I)> | 12 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 1160 | 690 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 293 | 20% NaCl, 6% PEG 6000 |
