6GFR
cyanobacterial GAPDH with NAD
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 200 |
Detector technology | PIXEL |
Collection date | 2017-02-27 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.96861 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 139.865, 139.865, 80.501 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 69.930 - 1.919 |
R-factor | 0.1669 |
Rwork | 0.165 |
R-free | 0.20630 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4boy |
RMSD bond length | 0.007 |
RMSD bond angle | 0.902 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 69.930 | 1.988 |
High resolution limit [Å] | 1.919 | 1.919 |
Rmerge | 0.221 | 0.923 |
Rmeas | 0.230 | 0.963 |
Number of reflections | 61333 | |
<I/σ(I)> | 7.94 | 2.03 |
Completeness [%] | 99.9 | 99.08 |
Redundancy | 12.9 | 12.7 |
CC(1/2) | 0.995 | 0.543 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 290 | 12% PEG3350 2% Tacsimate, 100 mM Hepes, pH 7 |