6G77
RSK4 N-terminal Kinase Domain in complex with AMP-PNP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-06-30 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 0.9163 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 51.143, 113.916, 62.183 |
Unit cell angles | 90.00, 90.24, 90.00 |
Refinement procedure
Resolution | 46.656 - 2.499 |
R-factor | 0.1875 |
Rwork | 0.185 |
R-free | 0.23300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3g51 |
RMSD bond length | 0.047 |
RMSD bond angle | 1.359 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.660 | 2.588 |
High resolution limit [Å] | 2.499 | 2.499 |
Rmerge | 0.065 | 0.255 |
Rmeas | 0.092 | |
Number of reflections | 23781 | 2409 |
<I/σ(I)> | 9.31 | |
Completeness [%] | 9.0 | 96.98 |
Redundancy | 1.9 | 1.9 |
CC(1/2) | 0.992 | 0.828 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 291 | 20 % PEG 6000, 0.1 M TRIS pH8, 2 mM Zinc chloride |