6FMY

IMISX-EP of S-PepTSt

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-08-14
Detector	DECTRIS EIGER X 16M
Wavelength(s)	2.0664
Spacegroup name	C 2 2 21
格子定数 [Å]	101.220, 110.200, 111.470
格子定数 [度]	90.00, 90.00, 90.00

精密化法

残基	49.290 - 2.700
R因子	0.2102
Rwork	0.208
R-free	0.24610
Structure solution method	SAD
結合長の平均二乗偏差(RMSD) [Å]	0.003
結合角の平均二乗偏差(RMSD) [度]	0.705
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELXCD
Refinement software	PHENIX ((1.13_2998: ???))

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	49.290	2.770
分解能 [Å] (高)	2.700	2.700
Rmeas	0.320	1.690
独立反射数	33070	2441
<I/σ(I)>	40.2	8.8
完全性 [%]	100.0	100
冗長性	423.6	378
CC(1/2)	1.000	0.960

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	LIPIDIC CUBIC PHASE		293	22 %(v/v) PEG- 400, 325 mM ammonium dihydrogen phosphate, 100 mM HEPES, pH 7.0