6D64

Crystal Structure of Human CD1b in Complex with POPC

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-06-27
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.95370
Spacegroup name	P 21 21 21
格子定数 [Å]	57.981, 80.032, 92.766
格子定数 [度]	90.00, 90.00, 90.00

精密化法

残基	40.130 - 1.700
R因子	0.18
Rwork	0.179
R-free	0.21100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5wl1
結合長の平均二乗偏差(RMSD) [Å]	0.008
結合角の平均二乗偏差(RMSD) [度]	1.053
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	46.950	1.730
分解能 [Å] (高)	1.700	1.700
Rpim	0.004	0.063
独立反射数	48206	2488
<I/σ(I)>	17.7	1.9
完全性 [%]	99.9	99.8
冗長性	14.7	14.8
CC(1/2)	0.999	0.675

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, HANGING DROP		293	PEG 3350, 0.2M Sodium Iodide, Ethylene Glycol