6BPR
Crystal structure of cysteine, nitric oxide-bound ferrous form of the uncrosslinked F2-Tyr157 human cysteine dioxygenase
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-10-13 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.97919 |
| Spacegroup name | P 65 |
| 格子定数 [Å] | 131.306, 131.306, 34.175 |
| 格子定数 [度] | 90.00, 90.00, 120.00 |
精密化法
| 残基 | 32.827 - 1.960 |
| R因子 | 0.1628 |
| Rwork | 0.161 |
| R-free | 0.18800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ic1 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Quality characteristics
| Overall | Inner shell | Outer shell | |
| 分解能 [Å] (低) | 50.000 | 50.000 | 1.990 |
| 分解能 [Å] (高) | 1.960 | 5.320 | 1.960 |
| Rmerge_l_obs | 0.143 | 0.058 | 0.999 |
| Total number of observations | 273552 | ||
| 独立反射数 | 24681 | 1326 | 1224 |
| <I/σ(I)> | 5 | ||
| 完全性 [%] | 100.0 | 99.7 | 100 |
| 冗長性 | 11.1 | 10.2 | 11 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | 0.1 M MES, 2 M ammonium sulfate, 2% PEG400 |
