6BL6
Crystallization of lipid A transporter MsbA from Salmonella typhimurium
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL7-1 |
Synchrotron site | SSRL |
Beamline | BL7-1 |
Temperature [K] | 80 |
Detector technology | CCD |
Collection date | 2016-01-28 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.127085 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 82.052, 154.819, 228.886 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.010 - 2.800 |
R-factor | 0.27104 |
Rwork | 0.270 |
R-free | 0.30071 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3b5w |
RMSD bond length | 0.009 |
RMSD bond angle | 1.368 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.010 | 2.850 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.136 | 0.136 |
Rmeas | 0.148 | |
Rpim | 0.057 | 0.057 |
Number of reflections | 60504 | |
<I/σ(I)> | 10 | |
Completeness [%] | 84.9 | 82 |
Redundancy | 5 | 4.1 |
CC(1/2) | 0.893 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | MsbA purified in detergent micelles (UDM/FA231) is mixed with lipid A prior to crystallization and mixed 1: 1 with well solution 20 mM Tris-Hcl (pH 7.5) and 15-20 5 PEG 300 . Crystals appeared in month (approx.) |