6AVS
Complex structure of JMJD5 and Symmetric Monomethyl-Arginine (MMA)
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-03-23 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 21 21 21 |
| 格子定数 [Å] | 49.685, 65.063, 78.139 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 50.000 - 2.020 |
| R因子 | 0.244 |
| Rwork | 0.241 |
| R-free | 0.27200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5fbj |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.026 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.917 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Quality characteristics
| Overall | |
| 分解能 [Å] (低) | 49.999 |
| 分解能 [Å] (高) | 2.000 |
| 独立反射数 | 17227 |
| <I/σ(I)> | 10.64 |
| 完全性 [%] | 99.0 |
| 冗長性 | 6.5 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 0.1M HEPES pH 7.0 8% PEG 3350 |
