6A8S

Crystal Structure of the putative amino acid-binding periplasmic ABC transporter protein from Candidatus Liberibacter asiaticus in complex with Cysteine

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-03
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.976251
Spacegroup name	P 21 21 21
格子定数 [Å]	45.791, 87.049, 122.164
格子定数 [度]	90.00, 90.00, 90.00

精密化法

残基	36.885 - 2.050
R因子	0.2001
Rwork	0.197
R-free	0.25830
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2yln
結合長の平均二乗偏差(RMSD) [Å]	0.007
結合角の平均二乗偏差(RMSD) [度]	0.886
Data reduction software	XDS (version June1, 2017)
Data scaling software	Aimless (0.5.32)
Phasing software	MoRDa (22(2018-05-05))
Refinement software	PHENIX ((1.13_2998: ???))

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	70.890	2.120
分解能 [Å] (高)	2.050	2.050
Rmerge_l_obs	0.147
Rmeas	0.147
Rpim	0.071	0.452
独立反射数	31433	3028
<I/σ(I)>	8.5	2.1
完全性 [%]	99.8	99.7
冗長性	5.2	5.3
CC(1/2)	0.994	0.702

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, SITTING DROP	5.5	277	2M Ammonium sulphate, 0.1M Sodium acetate Trihydrate