6A80
Crystal Structure of putative amino acid binding periplasmic ABC transporter protein from Candidatus Liberibacter asiaticus in complex with cystine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID30B |
Synchrotron site | ESRF |
Beamline | ID30B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-11-03 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.976251 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 46.160, 86.780, 122.250 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 70.760 - 1.560 |
R-factor | 0.18731 |
Rwork | 0.185 |
R-free | 0.22131 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2yln |
RMSD bond length | 0.020 |
RMSD bond angle | 2.091 |
Data reduction software | XDS (Version June 1, 2017) |
Data scaling software | autoPROC (1.1.7) |
Phasing software | MoRDa (22 (2018-05-05)) |
Refinement software | REFMAC (5.8.0218) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 70.760 | 1.587 |
High resolution limit [Å] | 1.560 | 1.560 |
Rmerge | 0.033 | 0.683 |
Rmeas | 0.038 | 0.781 |
Rpim | 0.018 | 0.500 |
Number of reflections | 70624 | 3510 |
<I/σ(I)> | 20.1 | 2.2 |
Completeness [%] | 99.8 | 99.7 |
Redundancy | 4.5 | 4.2 |
CC(1/2) | 0.999 | 0.809 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 277 | 2M Ammonium sulphate, 0.1M Sodium acetate Trihydrate |