6A6N
Crystal structure of an inward-open apo state of the eukaryotic ABC multidrug transporter CmABCB1
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-12-19 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | H 3 2 |
| 格子定数 [Å] | 179.690, 179.690, 157.666 |
| 格子定数 [度] | 90.00, 90.00, 120.00 |
精密化法
| 残基 | 47.140 - 3.020 |
| R因子 | 0.22823 |
| Rwork | 0.226 |
| R-free | 0.27473 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3wme |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.010 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.490 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 47.140 | 3.100 |
| 分解能 [Å] (高) | 3.020 | 3.020 |
| 独立反射数 | 19238 | |
| <I/σ(I)> | 14.3 | |
| 完全性 [%] | 99.6 | |
| 冗長性 | 9.98 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 14% PEG 2000 MME, 100mM magnesium nitrate |
