6A5K
Crystal structure of Arabidopsis thaliana SUVH6 in complex with SAM, form 1
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-01-09 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.2827 |
| Spacegroup name | P 21 21 2 |
| 格子定数 [Å] | 77.113, 122.152, 56.315 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 47.878 - 1.900 |
| R因子 | 0.1856 |
| Rwork | 0.184 |
| R-free | 0.21400 |
| Structure solution method | SAD |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.005 |
| 結合角の平均二乗偏差(RMSD) [度] | 0.945 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 50.000 | 1.930 |
| 分解能 [Å] (高) | 1.900 | 1.900 |
| Rmerge_l_obs | 0.071 | 0.874 |
| 独立反射数 | 42734 | 2102 |
| <I/σ(I)> | 41.6 | 2.4 |
| 完全性 [%] | 99.9 | 99.8 |
| 冗長性 | 16.9 | 10.9 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | 0.2M potassium sodium tartrate, 20% PEG 3350 |
