5ZME
Nucleotide-free form of C. reinhardtii ArsA1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL15A1 |
Synchrotron site | NSRRC |
Beamline | BL15A1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-02-25 |
Detector | RAYONIX MX300HE |
Wavelength(s) | 1.000 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 91.035, 91.035, 307.510 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.861 - 3.603 |
R-factor | 0.2911 |
Rwork | 0.288 |
R-free | 0.31910 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.606 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX ((1.11_2567: ???)) |
Refinement software | PHENIX ((1.11_2567: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.860 | 3.730 |
High resolution limit [Å] | 3.600 | 3.600 |
Number of reflections | 15761 | |
<I/σ(I)> | 29.9 | |
Completeness [%] | 99.5 | |
Redundancy | 7.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 100 mM Sodium HEPES pH7.5, 20% Glycerol, 10% PEG6000 and 3% 2-Methyl-2,4-pentanediol |