5YSB
Crystal structure of beta-1,2-glucooligosaccharide binding protein in ligand-free form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-06-28 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 1.0000 |
Spacegroup name | P 32 |
Unit cell lengths | 74.925, 74.925, 120.844 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.240 - 2.200 |
R-factor | 0.19246 |
Rwork | 0.190 |
R-free | 0.24679 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3uor |
RMSD bond length | 0.016 |
RMSD bond angle | 1.667 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.240 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.097 | 0.571 |
Rpim | 0.047 | 0.263 |
Number of reflections | 38141 | 1912 |
<I/σ(I)> | 21 | |
Completeness [%] | 98.8 | 100 |
Redundancy | 5.5 | 5.7 |
CC(1/2) | 0.906 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.15M zinc acetate, 15% (w/v) PEG 3350 |