5YO5
Crystal Structure of B562RIL with engineered disulfide bond A20C-Q25C
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-08-18 |
Detector | MAR CCD 130 mm |
Wavelength(s) | 0.9793 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 71.665, 120.870, 95.255 |
Unit cell angles | 90.00, 90.04, 90.00 |
Refinement procedure
Resolution | 37.700 - 2.200 |
R-factor | 0.2226 |
Rwork | 0.221 |
R-free | 0.26730 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1m6t |
RMSD bond length | 0.008 |
RMSD bond angle | 0.926 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.10_2155) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 37.700 |
High resolution limit [Å] | 2.200 |
Number of reflections | 79301 |
<I/σ(I)> | 12.35 |
Completeness [%] | 99.0 |
Redundancy | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 292 | 3.2M NH4(SO4), 0.1M Bicine 2.44mM N-octanoylsucrose |