5YAB
Crystal structure of scyllo-inositol dehydrogenase with L-glucose dehydrogenase activity
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2010-10-09 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 21 |
| 格子定数 [Å] | 90.974, 127.453, 137.535 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 28.300 - 1.750 |
| R因子 | 0.17734 |
| Rwork | 0.177 |
| R-free | 0.19184 |
| Structure solution method | SAD |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.009 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.356 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0107) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 50.000 | 1.810 |
| 分解能 [Å] (高) | 1.750 | 1.750 |
| Rmerge_l_obs | 0.055 | 0.247 |
| 独立反射数 | 160030 | |
| <I/σ(I)> | 24.7 | 4.9 |
| 完全性 [%] | 99.3 | 94.4 |
| 冗長性 | 3.7 | 3.1 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 12 - 35% PEG3350, 0.1 M sodium acetate, pH 4.8 - 5.4 |
