5XJ8
Crystal structure of PlsY (YgiH), an integral membrane glycerol 3-phosphate acyltransferase - the lysphosphatidic acid form
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-01-11 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97915 |
| Spacegroup name | C 1 2 1 |
| 格子定数 [Å] | 123.888, 40.895, 91.705 |
| 格子定数 [度] | 90.00, 95.59, 90.00 |
精密化法
| 残基 | 35.330 - 2.410 |
| R因子 | 0.23 |
| Rwork | 0.228 |
| R-free | 0.26350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.002 |
| 結合角の平均二乗偏差(RMSD) [度] | 0.459 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575)) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 35.330 | 2.500 |
| 分解能 [Å] (高) | 2.410 | 2.410 |
| Rmerge_l_obs | 0.099 | 0.750 |
| Rpim | 0.060 | 0.448 |
| 独立反射数 | 18124 | |
| <I/σ(I)> | 11.37 | 2 |
| 完全性 [%] | 99.1 | 96.8 |
| 冗長性 | 3.7 | 3.7 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | LIPIDIC CUBIC PHASE | 8 | 293 | 1mol% of palmitoyl lysophosphatidic acid, 39% PEG 350 MME, 0.04M NaCl, 0.04M Tris HCl pH 8.0 |
