5XF7

Crystal structure of human protein disulfide isomerase-like protein of the testis

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-11-11
Detector	APEX II CCD
Wavelength(s)	0.987
Spacegroup name	P 32
格子定数 [Å]	97.361, 97.361, 57.588
格子定数 [度]	90.00, 90.00, 120.00

精密化法

残基	28.106 - 2.381
R因子	0.2003
Rwork	0.197
R-free	0.23520
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ekz 4nwy
結合長の平均二乗偏差(RMSD) [Å]	0.008
結合角の平均二乗偏差(RMSD) [度]	0.957
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	50.000	2.420
分解能 [Å] (高)	2.380	2.380
独立反射数	24401
<I/σ(I)>	20.6	2
完全性 [%]	99.8	100
冗長性	3.8	3.8

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, HANGING DROP	7	289	21% (w/v) PEG 3350 and 0.1 M succinic acid, pH 7.0