5WWW
Crystal structure of the KH1 domain of human RNA-binding E3 ubiquitin-protein ligase MEX-3C complex with RNA
Experimental procedure
実験手法 | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-12-12 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.979 |
Spacegroup name | P 62 |
格子定数 [Å] | 76.366, 76.366, 36.392 |
格子定数 [度] | 90.00, 90.00, 120.00 |
精密化法
残基 | 38.183 - 1.798 |
R因子 | 0.1738 |
Rwork | 0.172 |
R-free | 0.20910 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2dgr |
結合長の平均二乗偏差(RMSD) [Å] | 0.006 |
結合角の平均二乗偏差(RMSD) [度] | 0.778 |
Data reduction software | MOSFLM |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.10_2155) |
Quality characteristics
Overall | Inner shell | Outer shell | |
分解能 [Å] (低) | 40.000 | 40.000 | 1.860 |
分解能 [Å] (高) | 1.800 | 3.880 | 1.800 |
Rmerge_l_obs | 0.159 | 0.121 | 0.705 |
Rmeas | 0.168 | 0.128 | 0.742 |
Rpim | 0.053 | 0.042 | 0.229 |
Total number of observations | 115295 | ||
独立反射数 | 11406 | ||
<I/σ(I)> | 3.6 | ||
完全性 [%] | 99.6 | 99.4 | 98.9 |
冗長性 | 10.1 | 9.6 | 10.3 |
CC(1/2) | 0.989 | 0.865 |
結晶化条件
結晶ID | 方法 | pH | 温度 | 溶液条件 |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 5% v/v Tacsimate pH 7.0, 0.1M HEPES pH 7.0, 10% w/v polyethylene glycol monomethyl ether 2000 |