5Q04
Human liver fructose-1,6-bisphosphatase 1 (fructose 1,6-bisphosphate 1-phosphatase, E.C.3.1.3.11) complexed with the allosteric inhibitor 1-(4-bromo-5-chlorothiophen-2-yl)sulfonyl-3-(5-bromo-1,3-thiazol-2-yl)urea
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-10-17 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 1 21 1 |
| 格子定数 [Å] | 66.281, 286.676, 83.272 |
| 格子定数 [度] | 90.00, 97.68, 90.00 |
精密化法
| 残基 | 29.235 - 2.502 |
| R因子 | 0.189 |
| Rwork | 0.186 |
| R-free | 0.25110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.008 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.069 |
| Refinement software | PHENIX (1.11.1_2575) |
Quality characteristics
| Overall | |
| 分解能 [Å] (低) | 29.235 |
| 分解能 [Å] (高) | 2.502 |
| 独立反射数 | 100143 |
| 完全性 [%] | 94.9 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 300 | 0.1M HEPES pH 7.0, 0.1M AMMONIUM ACETATE, 12%-25% PEG 3350 |
