5PZT

Human liver fructose-1,6-bisphosphatase 1 (fructose 1,6-bisphosphate 1-phosphatase, E.C.3.1.3.11) complexed with the allosteric inhibitor 1-(5-bromo-1,3-thiazol-2-yl)-3-(3-ethyl-4-phenylphenyl)sulfonylurea

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	CCD
Collection date	2005-12-06
Detector	MAR CCD 165 mm
Wavelength(s)	0.987
Spacegroup name	P 1 21 1
格子定数 [Å]	66.435, 294.192, 83.569
格子定数 [度]	90.00, 97.56, 90.00

精密化法

残基	29.501 - 2.800
R因子	0.196
Rwork	0.193
R-free	0.26120
Structure solution method	MOLECULAR REPLACEMENT
結合長の平均二乗偏差(RMSD) [Å]	0.009
結合角の平均二乗偏差(RMSD) [度]	1.099
Refinement software	PHENIX (1.11.1_2575)

Quality characteristics

	Overall
分解能 [Å] (低)	29.501
分解能 [Å] (高)	2.800
独立反射数	77696
完全性 [%]	99.9

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, SITTING DROP	7	300	0.1M HEPES pH 7.0, 0.1M AMMONIUM ACETATE, 12%-25% PEG 3350