5MME
Crystal structure of CREBBP bromodomain complexd with US46C
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-09-26 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.99998 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 53.371, 54.265, 81.048 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.090 - 1.350 |
R-factor | 0.1641 |
Rwork | 0.164 |
R-free | 0.17610 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3dwy |
RMSD bond length | 0.005 |
RMSD bond angle | 0.884 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 45.090 |
High resolution limit [Å] | 1.350 |
Rmerge | 0.048 |
Number of reflections | 51995 |
<I/σ(I)> | 17.2 |
Completeness [%] | 99.2 |
Redundancy | 6.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | 0.1 M Cacodylate, pH6.5, 0.2 M Calcium acetate, 18% PEG8000 |