5LN5
Crystal structure of the Wss1 E203Q mutant from S. pombe
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-06-18 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| 格子定数 [Å] | 41.160, 57.260, 50.640 |
| 格子定数 [度] | 90.00, 113.01, 90.00 |
精密化法
| 残基 | 15.000 - 1.750 |
| R因子 | 0.17404 |
| Rwork | 0.173 |
| R-free | 0.19541 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5jig |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.005 |
| 結合角の平均二乗偏差(RMSD) [度] | 0.965 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 30.000 | 1.850 |
| 分解能 [Å] (高) | 1.750 | 1.750 |
| Rmerge_l_obs | 0.041 | 0.431 |
| 独立反射数 | 20977 | |
| <I/σ(I)> | 14.1 | 2.7 |
| 完全性 [%] | 95.3 | 94.6 |
| 冗長性 | 3.5 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 100 mM TRIS/HCl, 20% PEG 1000 |
