5L0C

Human metavinculin (residues 959-1134) in complex with PIP2

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2012-06-28
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97
Spacegroup name	C 1 2 1
格子定数 [Å]	114.520, 100.676, 105.615
格子定数 [度]	90.00, 122.49, 90.00

精密化法

残基	30.450 - 3.100
R因子	0.2368
Rwork	0.235
R-free	0.26060
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3myi
結合長の平均二乗偏差(RMSD) [Å]	0.010
結合角の平均二乗偏差(RMSD) [度]	1.130
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	BUSTER (2.10.1)

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	89.080	3.270
分解能 [Å] (高)	3.100	3.100
Rpim	0.034
独立反射数	18132
<I/σ(I)>	15.2	2.1
完全性 [%]	98.2	100
冗長性	3.7	3.8
CC(1/2)	0.999	0.742

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, HANGING DROP		294	20 mM citric acid, 80 mM Bis-Tris propane (pH 3.4), and 16% (w/v) PEG 3350