5JA5

Crystal structure of the rice Topless related protein 2 (TPR2) N-terminal topless domain (1-209) L111A and L130A mutant in complex with rice D53 repressor EAR peptide motif

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2015-08-07
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.078
Spacegroup name	P 42 21 2
Unit cell lengths	57.317, 57.317, 173.483
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.612 - 2.000
R-factor	0.2043
Rwork	0.203
R-free	0.22780
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4zhe
RMSD bond length	0.005
RMSD bond angle	0.847
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.050
High resolution limit [Å]	2.000	2.000
Rmerge	0.070	0.893
Number of reflections	20502
<I/σ(I)>	25.9	4
Completeness [%]	100.0	100
Redundancy	15.6	16
CC(1/2)	1.000

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.8	293	20% w/v Polyethylene glycol 3350, 0.2 M Potassium phosphate monobasic, pH 4.8