5J2Y
Molecular insight into the regulatory mechanism of the quorum-sensing repressor RsaL in Pseudomonas aeruginosa
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | OTHER |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-10-30 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.97915 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 97.245, 52.983, 69.809 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.930 - 2.400 |
R-factor | 0.22499 |
Rwork | 0.224 |
R-free | 0.25182 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.357 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AutoSol |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.087 | 0.489 |
Number of reflections | 13851 | |
<I/σ(I)> | 12.2 | 1.6 |
Completeness [%] | 99.5 | 97.8 |
Redundancy | 3.4 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.2 M Sodium acetate trihydrate, 0.1 M Tris hydrochloride pH8.5, 30% Polyethylene glycol 4000 |