5J2Y

Molecular insight into the regulatory mechanism of the quorum-sensing repressor RsaL in Pseudomonas aeruginosa

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	OTHER
Temperature [K]	100
Detector technology	CCD
Collection date	2015-10-30
Detector	ADSC QUANTUM 4
Wavelength(s)	0.97915
Spacegroup name	P 21 21 2
格子定数 [Å]	97.245, 52.983, 69.809
格子定数 [度]	90.00, 90.00, 90.00

精密化法

残基	39.930 - 2.400
R因子	0.22499
Rwork	0.224
R-free	0.25182
Structure solution method	SAD
結合長の平均二乗偏差(RMSD) [Å]	0.009
結合角の平均二乗偏差(RMSD) [度]	1.357
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	AutoSol
Refinement software	REFMAC (5.7.0029)

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	40.000	2.490
分解能 [Å] (高)	2.400	2.400
Rmerge_l_obs	0.087	0.489
独立反射数	13851
<I/σ(I)>	12.2	1.6
完全性 [%]	99.5	97.8
冗長性	3.4	3

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	0.2 M Sodium acetate trihydrate, 0.1 M Tris hydrochloride pH8.5, 30% Polyethylene glycol 4000