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5J2Y

Molecular insight into the regulatory mechanism of the quorum-sensing repressor RsaL in Pseudomonas aeruginosa

Experimental procedure
実験手法SINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsOTHER
Temperature [K]100
Detector technologyCCD
Collection date2015-10-30
DetectorADSC QUANTUM 4
Wavelength(s)0.97915
Spacegroup nameP 21 21 2
格子定数 [Å]97.245, 52.983, 69.809
格子定数 [度]90.00, 90.00, 90.00
精密化法
残基39.930 - 2.400
R因子0.22499
Rwork0.224
R-free0.25182
Structure solution methodSAD
結合長の平均二乗偏差(RMSD) [Å]0.009
結合角の平均二乗偏差(RMSD) [度]1.357
Data reduction softwareHKL-2000
Data scaling softwareHKL-2000
Phasing softwareAutoSol
Refinement softwareREFMAC (5.7.0029)
Quality characteristics
 OverallOuter shell
分解能 [Å] (低)40.0002.490
分解能 [Å] (高)2.4002.400
Rmerge_l_obs0.0870.489
独立反射数13851
<I/σ(I)>12.21.6
完全性 [%]99.597.8
冗長性3.43
結晶化条件
結晶ID方法pH温度溶液条件
1VAPOR DIFFUSION, HANGING DROP8.52930.2 M Sodium acetate trihydrate, 0.1 M Tris hydrochloride pH8.5, 30% Polyethylene glycol 4000

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件を2024-09-18に公開中

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