5IQP

14-3-3 PROTEIN TAU ISOFORM

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID2
Synchrotron site	ESRF
Beamline	ID2
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1995-04-15
Detector	MARRESEARCH
Wavelength(s)	0.87
Spacegroup name	P 21 21 21
格子定数 [Å]	70.290, 79.300, 101.000
格子定数 [度]	90.00, 90.00, 90.00

精密化法

残基	14.967 - 2.602
R因子	0.1891
Rwork	0.186
R-free	0.23930
Structure solution method	MIR
結合長の平均二乗偏差(RMSD) [Å]	0.002
結合角の平均二乗偏差(RMSD) [度]	0.398
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	PHENIX ((1.10.1_2155: ???))

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	14.967	2.690
分解能 [Å] (高)	2.602	2.602
Rmerge_l_obs	0.065	0.295
独立反射数	17742
<I/σ(I)>	15.9
完全性 [%]	99.7
冗長性	3.2

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, HANGING DROP	6.4	291	24% PEG4K, 140 mM LI2SO4, 4 % of SATURATED NACL IN 80 mM MES AT pH 6.4; PROTEIN 7 mg/ml