5IK9
Tobacco 5-epi-aristolochene with farnesyl monophosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-06-06 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 126.440, 126.440, 123.530 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 56.546 - 2.230 |
| R-factor | 0.1554 |
| Rwork | 0.154 |
| R-free | 0.18830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5im1 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.970 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.550 | 2.310 |
| High resolution limit [Å] | 2.230 | 2.230 |
| Rmerge | 0.105 | 0.532 |
| Number of reflections | 49350 | |
| <I/σ(I)> | 14.57 | 4.29 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 9.5 | 9.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 278 | 100 mM MOPSO, pH 7, 300 mM Mg Acetate, 15% PEG 8000 |
