5HV9
Human LTC4S mutant-S36E
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-12-15 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9795 |
| Spacegroup name | F 2 3 |
| Unit cell lengths | 168.042, 168.042, 168.042 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.010 - 3.000 |
| R-factor | 0.29032 |
| Rwork | 0.289 |
| R-free | 0.31192 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2uui |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.361 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.010 | 3.108 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.090 | |
| Number of reflections | 7999 | |
| <I/σ(I)> | 20.57 | 0.99 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 13.4 | 13.9 |
| CC(1/2) | 1.000 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 2.0 M NH4SO4, 0.2 M NaCl, 0.1 M Na-cacodylate, pH 6.5 |
