5H5L
Structure of prostaglandin synthase D of Nilaparvata lugens
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-01-16 |
Detector | RAYONIX MX300HE |
Wavelength(s) | 0.9 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 60.043, 72.036, 93.362 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.351 - 1.999 |
R-factor | 0.1763 |
Rwork | 0.173 |
R-free | 0.23800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3vpq |
RMSD bond length | 0.011 |
RMSD bond angle | 1.078 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | HKL-2000 |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.400 | 2.030 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 28052 | |
<I/σ(I)> | 32 | |
Completeness [%] | 99.9 | |
Redundancy | 7.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293.15 | 0.1 M Tris-HCl, 28% PEG4000 (w/v) |