5GWP

Crystal structure of RCAR3:PP2C wild-type with (+)-ABA

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2015-10-18
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9795
Spacegroup name	C 2 2 21
格子定数 [Å]	74.981, 133.684, 191.279
格子定数 [度]	90.00, 90.00, 90.00

精密化法

残基	46.136 - 2.577
R因子	0.2087
Rwork	0.206
R-free	0.24670
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3rt0
結合長の平均二乗偏差(RMSD) [Å]	0.002
結合角の平均二乗偏差(RMSD) [度]	0.519
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.10_2155: ???))

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	46.144	2.670
分解能 [Å] (高)	2.577	2.577
Rmerge_l_obs	0.114
独立反射数	30728
<I/σ(I)>	22.02
完全性 [%]	96.0
冗長性	11
CC(1/2)	0.997

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, HANGING DROP	6	295	PEG 8000, MES pH 6.0, calcium acetate